recombinant shhn  (R&D Systems)

Journal: bioRxiv

Article Title: Scube2 primes Dispatched and ADAM10-mediated Shh release by recruiting HDL acceptors to the plasma membrane

doi: 10.1101/2025.01.20.633902

Figure Lengend Snippet: A-C) Solubilization of non-palmitoylated C25S Shh, non-cholesteroylated ShhN and non-lipidated C25S ShhN in serum-depleted media. C25S Shh release remains associated with A10 function but is less controlled by Scube2. ShhN release is independent of A10 and Scube2, as is the solubilization of the C25S ShhN control. A’-C’) Protein quantification from HEK cells or A10 -/- cells in the presence of Scube2. Shh signals from HEK cells also expressing Scube2 were set to 100%. Unpaired t-test, two-tailed. ns: p>0.05, ****: p<0.0001, n=7 (A’), n=6 (B’), n=8 (C’). A’’-C’’) Quantification of protein solubilization from HEK cells in the presence or absence of Scube2. Shh signals from HEK cells also expressing Scube2 were set to 100%. Unpaired t-test, two-tailed. ns: p>0.05, *: p<0.05, n=7 (A’’), n=3 (B’’), n=8 (C’’). See Supplementary Table 1 for detailed statistical information.

Article Snippet: Unlipidated C25S ShhN cDNA and non-palmitoylated C25S Shh cDNA (amino acids 1-438) were inserted into pcDNA3.1 (Invitrogen).

Techniques: Control, Expressing, Two Tailed Test

Journal: eLife

Article Title: Two-way Dispatched function in Sonic hedgehog shedding and transfer to high-density lipoproteins

doi: 10.7554/eLife.86920

Figure Lengend Snippet:

Article Snippet: Recombinant DNA reagent , ShhN/ C25A/S Shh , Grobe Lab , OriGene NM_009170 , Monolipidated Shh.

Techniques: Variant Assay, Expressing, Knock-Out, Control, Transfection, Construct, Plasmid Preparation, Recombinant, Functional Assay, Software, Western Blot, Derivative Assay, Binding Assay, Immunoprecipitation